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Meridian Bioscience 10x reaction buffer 10x nh 4
10x Reaction Buffer 10x Nh 4, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/10x+nh+4+reaction+buffer/10%C3%97+nh4+reaction+buffer/pmc11542218-92-35-42
Average 90 stars, based on 1 article reviews
10x reaction buffer 10x nh 4 - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: The Genus Cerion (Gastropoda: Cerionidae) in the Florida Keys
Article Snippet: .. PCR amplifications were performed in 20 μL volumes that contained: 12.9 μL ddH 2 O, 2 μL 10x NH 4 reaction buffer (Bioline) 1.2μL of 50mM MgCl 2 (Bioline), 1 μL dNTPs, 0.5 μL of 100x BSA (BioLab), 0.6 μL of 10μM forward primer (Integrated DNA Technologies, Inc.), 0.6 μL of 10μM reverse primer (Integrated DNA Technologies, Inc.), 0.2 μL of Biolase DNA polymerase (Bioline) and 1 μL of extracted genomic DNA. .. For COI, the amplification protocol for the thermocycler (Applied Biosystems, Life Technologies) was as follows: initial denaturation at 95°C for 3 minutes, followed by 45 cycles of denaturation at 94°C for 30 seconds, annealing at 45°C for 45 seconds and extension at 72°C for 2 minutes, with a final extension at 72°C for 5 minutes.



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